Method of producing organotypic cell cultures

C - Chemistry – Metallurgy – 12 – N

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C12N 5/07 (2010.01) C12M 3/00 (2006.01)

Patent

CA 2612269

The present invention relates to cell and tissue culture. In particular, the present invention provides a method for preparing an organotypic culture using dissociated cells or microexplants obtained from an animal organ. The method for preparing an organotypic culture comprises culturing cells from an organ on a surface characterised in that the cells are compacted. The invention further relates to a high-throughput method for the preparation of a collection of organotypic cultures. The invention further relates to a device for carrying out a method of organotypic culture according to the invention.

L'invention concerne la culture cellulaire et tissulaire. L'invention concerne en particulier un procédé de préparation d'une culture organotypique au moyen de cellules ou de microexplants dissociés obtenus à partir d'un organe animal. Le procédé de préparation d'une culture organotypique consiste à cultiver des cellules issues d'un organe sur une surface, et est caractérisé en ce que les cellules sont compactées. L'invention concerne également un procédé à haut rendement pour la préparation d'une collection de cultures organotypiques. L'invention concerne en outre un dispositif permettant de mettre en oeuvre un procédé de préparation de culture organotypique de l'invention.

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