Method of site-specific insertion in zymomonas mobilis

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 1/21 (2006.01) C12N 15/11 (2006.01) C12N 15/63 (2006.01) C12N 15/74 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2304927

The present invention briefly provides a method of site-specific insertion in Zymomonas, comprising, providing a Zymomonas gene fragment, interrupting a DNA sequence the fragment, and transforming the Zymomonas through homologous recombination with the interrupted fragment.

L'invention décrit brièvement une méthode d'insertion spécifique du site dans Zymomonas, qui comprend l'obtention d'un fragment génique de Zymomonas, l'interruption d'une séquence d'ADN du fragment et la transformation de Zymomonas par recombinaison homologue avec le fragment interrompu.

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