Methods and assays for capture of nucleic acids

Details Methods and assays for capture of nucleic acids Methods and assays for capture of nucleic acids

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C40B 30/04 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2725405

The present disclo-sure provides methods and systems for sequence specific nucleic acid target capture comprising enzymatic reactions. The present disclosure re-lates to a plurality of oligonucleotide probes for capture and subsequent detection of target nucleic acid se-quences, using flap endonucleases, ligases, and/or additional enzymes, proteins or compounds, on sub-strates, for example microarray slides, and in solution formats.

La présente invention concerne des procédés et des systèmes comprenant des réactions enzymatiques pour la capture d'acides nucléiques cibles spécifiques d'une séquence. La présente invention concerne une pluralité de sondes oligonucléotidiques pour la capture suivie de la détection de séquences d'acides nucléiques cibles, utilisant des endonucléases de l'ADN flap, des ligases, et/ou des enzymes, des protéines ou des composés additionnels, sur des substrats, par exemple des lames en réseau, et sous forme de solution.

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