Methods and compositions for chromatography

Details Methods and compositions for chromatography Methods and compositions for chromatography

B - Operations, Transporting

01

D

B01D 15/08 (2006.01) G01N 30/50 (2006.01) B01D 15/18 (2006.01) G01N 30/38 (2006.01) G01N 30/60 (2006.01)

Patent

CA 2466664

The present invention is directed to methods and compositions for separating and isolating target molecules. In particular, the present invention comprises devices, such as CCDs, that contain particles without the need of support structures. Chromatography separation techniques, including but not limited to, ion exchange, reversed phase and normal phase partitioning, are used in the CCD. Methods also include low, medium and high pressure liquid chromatography. such methods can be used for analytical, semi-preparative processes, initial clarification, preparative filtration and process scale applications.

L'invention concerne des procédés et des compositions permettant de séparer et d'isoler des molécules cibles. L'invention concerne en particulier des dispositifs, de type CCD, qui contiennent des particules ne nécessitant pas de structures de support. Des techniques de séparation par chromatographie comprenant, de façon non exhaustive, la chromatographie d'échange d'ions, la chromatographie par séparation fondée sur la taille, la chromatographie d'affinité, la chromatographie d'exclusion d'ions, la chromatographie d'échange de ligands, la chromatographie de séparation inversée en phase liquide et en phase normale, sont mises en application dans le dispositif CCD. L'invention concerne également des procédés mettant en application une chromatographie liquide à faible, moyenne et haute pression. Lesdits procédés peuvent être mis en application dans des processus à l'échelle analytique, de semi-préparation, de clarification initiale, et de filtration et de traitement de préparation.

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