C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 7/52 (2006.01) C12N 15/00 (2006.01) C12P 7/40 (2006.01) C12N 1/20 (2006.01)
Patent
CA 2728262
A fermentation process for the production of organic acids is provided and includes, providing metabolically engi-neered mutant bacteria which have had one or both of ack and pta genes disrupted, adapting the mutant bacteria to increase their resistance to acids and to increase their growth rate by immobilizing the mutant bacteria while exposing the mutant bacteria to a fermentable substrate, and further exposing the adapted mutant bacteria to a fermentable substrate for a time sufficient to provide a final organic acid fermentation product concentration of greater than about 50 g/L.
Linvention concerne un procédé de fermentation pour la production dacides organiques. Ce procédé comprend lutilisation de bactéries mutantes métaboliquement modifiées qui possèdent un ou les deux gènes ack et pta interrompus, ladaptation des bactéries mutantes pour augmenter leur résistance aux acides et pour augmenter leur taux de croissance en immobilisant les bactéries mutantes tout en exposant les bactéries mutantes à un substrat fermentable, ainsi quune exposition supplémentaire des bactéries mutantes adaptées à un substrat fermentable pendant une durée suffisante pour obtenir une concentration finale de produit de fermentation dacides organiques supérieure à environ 50 g/l.
Gowling Lafleur Henderson Llp
The Ohio State University
LandOfFree
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