C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/04 (2006.01) C12Q 1/66 (2006.01) G01N 21/64 (2006.01) G01N 33/569 (2006.01)
Patent
CA 2741449
A process for measuring total microbiological content in an aqueous medium includes adding a fluorescent dye to the aqueous medium, measuring the fluorescent signal in the aqueous medium to obtain a baseline fluorescent signal, releasing intracellular content of the microbiological matter into the aqueous medium by lysing the microbiological matter, measuring the fluorescent signal in the aqueous medium with the released intracellular content of the microbiological matter to obtain a second fluorescent signal, subtracting the baseline signal from the second fluorescent signal to obtain a net fluorescent signal and equating the net fluorescent signal with a microbiological content. Systems for measuring total microbiological content and methods for measuring biofilm and adjusting for background noise are also provided.
L'invention porte sur un procédé de mesure de la teneur microbiologique totale dans un milieu aqueux, qui comprend les opérations consistant à ajouter un colorant fluorescent au milieu aqueux, à mesurer le signal fluorescent dans le milieu aqueux pour obtenir un signal fluorescent de référence, à libérer le contenu intracellulaire de la matière microbiologique dans le milieu aqueux par lyse de la matière microbiologique, à mesurer le signal fluorescent dans le milieu aqueux avec la teneur intracellulaire libérée de la matière microbiologique et pour obtenir un second signal fluorescent, à soustraire le signal de référence à partir du second signal fluorescent pour obtenir un signal fluorescent net et à faire correspondre le signal fluorescent net avec une teneur microbiologique. L'invention porte également sur des systèmes de mesure de la teneur microbiologique totale et des procédés pour mesurer un biofilm et ajuster le bruit de fond.
Boyette Scott Martell
Cai Hong
Hirst Paul Ronald
Jiang Juan
Jin Yan
Company General Electric
Craig Wilson And Company
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