C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/073 (2010.01) C12N 5/071 (2010.01) A01N 1/02 (2006.01) A61K 35/44 (2006.01) A61P 35/00 (2006.01)
Patent
CA 2652051
An innovative method of collecting cord blood stem cells from an isolated mammalian non-exsanguinated or partially exsanguinated placenta by placental perfusion is described. Placental perfusion can include perfusing the isolated placenta with a pulsatile flow of perfusion solution, for example, using a pulsatile or peristaltic pump or device. The stem cells can then be isolated from the perfusate. The perfusion solution can include an anticoagulant. The isolated mammalian placenta need not be treated with an anticoagulant prior to perfusing. The isolated placenta can be free from an anticoagulant prior to perfusing.
La présente invention concerne un nouveau procédé de prélèvement de cellules souches de sang de cordon ombilical provenant d'un placenta mammalien isolé, non exsanguiné ou partiellement exsanguiné, au moyen d'une perfusion placentaire. La perfusion placentaire peut consister à perfuser le placenta isolé avec un flux pulsatile de solution de perfusion, en utilisant par exemple une pompe ou un dispositif pulsatile ou péristaltique. Les cellules souches peuvent ensuite être isolées du perfusat. La solution de perfusion peut contenir un anticoagulant. Il n'est pas nécessaire de traiter le placenta mammalien isolé avec un anticoagulant avant la perfusion et le placenta isolé peut être exempt d'anticoagulant avant d'être perfusé.
Cord Blood Science Inc.
Riches Mckenzie & Herbert Llp
Takebe Naoko
LandOfFree
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