A - Human Necessities – 01 – N
Patent
A - Human Necessities
01
N
A01N 1/02 (2006.01) A61K 38/17 (2006.01) C12M 3/00 (2006.01) C12M 3/02 (2006.01) A61K 48/00 (2006.01) C12N 5/06 (2006.01)
Patent
CA 2364024
The present invention provides methods for culturing fluid-filled sensory organs in vitro. In particular, the present invention provides methods for culturing the eyeball, including the retina, and the inner ear, including the Organ of Corti, in vitro. The methods of the present invention involve little or no microsurgical dissection of the entire fluid-filled sensory organ, thereby preserving the structural and functional integrity of the sensory epithelium. The methods of the present invention include the steps of (a) introducing a fluid-filled sensory organ (such as an inner ear or an eye ball) into a culture chamber containing liquid culture medium and (b) moving the culture chamber so that the fluid-filled sensory organ moves within the culture chamber. Preferably the culture chamber is cylindrical or annular in shape and is rotated about its longitudinal axis.
La présente invention concerne un procédé permettant de cultiver in vitro des organes sensoriels remplis de fluide. L'invention concerne plus particulièrement des procédés permettant la culture in vitro du globe oculaire, y-compris la rétine, et de l'oreille interne, y-compris l'organe de Corti. Le procédé de l'invention n nécessite quasiment pas de dissection de petite chirurgie ou de microchirurgie de la totalité de l'organe sensoriel rempli de liquide, ce qui permet de préserver l'intégrité structurelle et fonctionnelle de l'épithélium sensoriel. Le procédé de l'invention comporte une première opération (a) consistant à introduire l'organe sensoriel rempli de fluide, notamment une oreille interne ou un globe oculaire, dans la chambre de culture contenant un milieu de culture liquide, et une seconde opération (b) consistant à remuer la chambre de culture de façon que l'organe sensoriel rempli de fluide bouge dans la chambre de culture. De préférence, la chambre de culture est de forme cylindrique ou annulaire, et vient tourner autour de son axe longitudinal.
Kil Jonathan
Lowenheim Hubert
Sudra Anish H.
Kil Jonathan
Lowenheim Hubert
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
Otogene Ag
Otogene Usa Inc.
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