Methods for detection and typing of nucleic acids

Details Methods for detection and typing of nucleic acids Methods for detection and typing of nucleic acids

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2610863

Disclosed are methods and kits for identifying and characterizing polynucleotide sequences in a sample which may include a heterogeneous sample. Some of the methods and kits are directed to the identification and characterization of a virus in a sample, which may include HIV capable of cause AIDS or AIDS-like symptoms. The virus may be HIV-1, and may also include drug resistant mutations. The methods may include reacting a mixture that includes, in addition to nucleic acid isolated from the sample, at least one oligonucleotide capable of specifically hybridizing to HIV nucleic acid where the oligonucleotide includes at least one non-natural base. In addition, the methods may include detection of one or more mutations in HIV nucleic acid that are associated with drug resistance.

L'invention concerne des méthodes et des kits d'identification et de caractérisation de séquences polynucléotidiques dans un échantillon pouvant être hétérogène. Certaines méthodes et kits de l'invention sont dirigés sur l'identification et la caractérisation d'un virus dans un échantillon, pouvant comprendre le VIH capable d'induire le SIDA ou des symptômes du type de ceux du SIDA. Le virus peut être le VIH-1 et peut également présenter des mutations résistantes aux médicaments. Ces méthodes consistent à faire réagir un mélange contenant, outre l'acide nucléique isolé dans l'échantillon, au moins un oligonucléotide capable de s'hybrider spécifiquement avec l'acide nucléique du VIH, l'oligonucléotide contenant au moins une base non naturelle. De plus, ces méthodes peuvent consister à détecter une ou plusieurs mutations dans l'acide nucléique du VIH, qui sont associées à la résistance aux médicaments.

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