Methods for determining the presence of nucleic acid target...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2359613

Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined exogenous nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, determination of viral load, genotyping, medical marker diagnostics, and the like.

La présente invention concerne des procédés dans lesquels il est fait appel à la dépolymérisation d'un hybride d'acide nucléique pour analyser qualitativement et quantitativement la présence d'un acide nucléique exogène prédéterminé. Les procédés de l'invention trouvent leur application, entre autres, dans la détection des polymorphismes de nucléotide unique, dans l'identification de changements affectant une seule base, dans la détermination de la charge virale, dans l'établissement de génotypes, dans le diagnostic au moyen de marqueurs médicaux et analogues.

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