C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/08 (2006.01) C12P 21/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/563 (2006.01)
Patent
CA 2390512
The present invention provides a method for producing proteins with a desired function, generally comprising the steps of (a) providing a population of antibody- forming cells suspected of containing at least one cell capable of producing an antibody exhibiting a desired function; (b) suspending the population of antibody- forming cells in a medium, the medium having an indicator system incorporated therein, the indicator system also being capable of indicating the presence and location of a cell which forms antibodies exhibiting the desired function; (c) identifying a cell forming an antibody exhibiting the desired function; (d) isolating the identified antibody-forming cell from the medium; (e) determining the amino acid sequence of the variable region or a portion thereof which confers the desired function of the antibody produced by the isolated antibody-forming cell; and (f) synthesizing a protein with a desired function, the protein containing the amino acid sequence of the variable region or portion thereof which confers the desired function.
Abgenix Biopharma Inc.
Gowling Lafleur Henderson Llp
LandOfFree
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