G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 1/40 (2006.01) C12M 1/34 (2006.01) C12M 1/38 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) G01N 35/00 (2006.01)
Patent
CA 2754884
Various embodiments of this invention provide a method of concentrating an analyte contained in a fluid sample provided to a receptacle having a plurality of interconnected chambers, the method comprising the steps of: (a) forming a first volume in a first chamber of the receptacle, the first volume comprising the fluid sample and a liquid reagent containing a solid support; (b) immobilizing the analyte on the solid support; (c) moving an aliquot of the first volume from the first chamber to a directly connected second chamber of the receptacle, wherein the volume capacity of the second chamber is less than the first volume; and (d) removing non-analyte components of the sample from the second chamber while retaining the analyte in the second chamber. Steps (c) through (d) are repeated while retaining the analyte in the second chamber thereby accumulating concentrated analyte in the second chamber.
Breidenthal Scott S.
Fan Sara H.
Lee Richard S.
Nelson Norman C.
Scott Matthew J.
Gen-Probe Incorporated
Qualigen Inc.
Smart & Biggar
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