Methods of creating genetic diversity

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/31 (2006.01) C12N 1/00 (2006.01) C12N 13/00 (2006.01) C12N 15/00 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12N 15/63 (2006.01) C12N 15/66 (2006.01) C12N 15/87 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2454248

The present invention relates to methods of creating genetic diversity .The invention also relates to methods of making nucleic acid libraries and their uses to screen for biological activities. The invention can be used to create, improve or modify nucleic acid sequences or repertoire, particularly from non- cultivable microorganisms and finds various applications in the medical, food, agriculture or veterinary industries, for instance. The invention particularly uses and discloses in situ gene shuffling.

La présente invention concerne des procédés destinés à créer une diversité génétique. L'invention a également pour objet des procédés destinés à produire des bibliothèques d'acide nucléique et leur utilisation pour le criblage d'activités biologiques. L'invention peut être utilisée pour créer, améliorer ou modifier des séquences d'acide nucléique et un répertoire, en particulier à partir de micro-organismes non cultivables, et trouvent différentes applications en médecine, dans l'industrie vétérinaire, l'industrie de l'alimentation et de l'agriculture, par exemple. L'invention concerne en particulier le remaniement génétique in situ et ses utilisations.

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