Methods of detecting gene expression in normal and cancerous...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) A61B 1/00 (2006.01) C07H 21/00 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2512956

The present invention provides methods for detecting gene expression in normal and cancerous cells. Specifically, provided are methods utilizing molecular beacons (MB) technology combined with fluorescence imaging techniques for detecting, identifying or quantitating the presence of, or alterations in gene expression of, various tumor markers in a sample of cells.

La présente invention concerne des procédés de détection d'une expression génique dans des cellules saines et dans des cellules cancéreuses. L'invention concerne plus particulièrement des procédés qui mettent en oeuvre une technologie de balises moléculaires (MB) combinée à des techniques d'imagerie par fluorescence pour détecter, identifier ou quantifier la présence ou des modifications d'expression génique de divers marqueurs tumoraux dans un échantillon de cellules.

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