Methods of inactivating pathogens using broad-spectrum...

A - Human Necessities – 61 – L

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A61L 2/08 (2006.01) A61L 2/00 (2006.01)

Patent

CA 2360131

Methods for reducing the pathogen content of a biologically derived composition by illuminating the composition with at least one high-intensity, short duration pulse of incoherent polychromatic light in a broad spectrum. In the resultant treated compositions, biomolecules of interest remain biologically active. The content of pathogens, such as viruses, bacteria, pyrogens, fungi and/or prions, present in a biologically derived composition, such as blood serum, blood plasma or other blood product, including, insulin, transferrin, heparin, collagen, Factor VIII and/or Factor IX, or a composition containing monoclonal antibodies, proteins from genetically engineered cell lines or the like, is reduced by illumination with broad-spectrum pulsed light. The content of HIV-1, SV40, canine parvovirus or bovine viral diarrhea virus in a biologically derived composition is dramatically reduced with a single pulse of broad-spectrum light.

La présente invention concerne un procédé permettant de réduire la teneur en pathogènes d'une composition biologiquement dérivée, et ce, en éclairant la composition d'au moins une impulsion de courte durée à forte intensité de lumière polychrome incohérente à large spectre. Dans les compositions traitées résultantes, les biomolécules intéressantes restent biologiquement actives. L'éclairage en lumière pulsée large spectre permet ainsi de réduire la teneur en pathogènes tels que les virus, bactéries, pyrogènes, champignons et/ou prions dans une composition biologiquement dérivée telle que le sérum, le plasma ou d'autres produits sanguins, y-compris l'insuline, la sidérophiline, l'héparine, le collagène, le Facteur VIII et/ou le Facteur IX, ou une composition contenant des anticorps monoclonaux, des protéines issues de lignées cellulaires obtenues par génie génétique ou analogues. Une seule impulsion de lumière large spectre suffit à faire baisser de façon considérable la teneur en VIH-1, SV40, parvovirus du chien ou virus diarrhée virale dans une composition biologiquement dérivée.

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