C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 19/34 (2006.01) A01N 43/04 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 9/22 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2291512
Methods for preparing nucleotide integrases are provided. The nucleotide integrases are prepared by combining in vitro an excised, group II intron RNA, referred to hereinafter as "exogenous RNA", with a group II intron-encoded protein. The exogenous RNA is prepared by in vitro transcription of a DNA molecule which comprises a group II intron sequence.
L'invention concerne des procédés visant à engendrer la nucléotide intégrase par association in vitro d'un ARN à intron du groupe II excisé, ci-après dénommé "ARN exogène", et d'une protéine à codage par intron du groupe II. La production d'ARN exogène résulte de la transcription in vitro d'une molécule d'ADN renfermant une séquence d'intron du groupe II.
Beall Clifford James
Guo Huatao
Lambowitz Alan M.
Matsuura Manabu
Mohr Georg
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
The Ohio State Research Foundation
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