C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/73, 530/3.08
C12N 15/00 (2006.01) C07K 1/113 (2006.01) C12N 9/64 (2006.01) C12N 9/72 (2006.01) C12P 21/00 (2006.01)
Patent
CA 1219234
ABSTRACT METHODS OF PURIFICATION AND REACTIVATION OF PRECIPITATED HETEROLOGOUS PROTEINS A heterologous expression product in host cells is separated from the host cell material by suspending the material in a buffered solution, disrupting the cells and separating the product as refractile material by low speed centrifugation. The refractile material can be dissolved in a strongly denaturing solution, which is then weakened while keeping the protein in solution, thereby allowing unfolding and refolding of the protein. Disulfide linkages in the protein may be cleaved, and reformed later after the protein has refolded.
443903
Builder Stuart E.
Jones Andrew J.s.
Lin Norm S.c.
Ogez John R.
Olson Kenneth C.
Genentech Inc.
Gowling Lafleur Henderson Llp
LandOfFree
Methods of purification and reactivation of precipitated... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods of purification and reactivation of precipitated..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods of purification and reactivation of precipitated... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1286941