Microfluidic apparatus and method for preparing cytological...

G - Physics – 01 – N

Patent

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G01N 1/31 (2006.01) G01N 1/40 (2006.01) G01N 15/06 (2006.01)

Patent

CA 2737593

An apparatus for processing a specimen from a fluid sample includes a first set of one or more microfluidic channels configured to deliver the sample fluid to a filter disposed on an inflatable bladder configured to transfer the specimen from the filter to a slide. The apparatus is configured to collect an approximate monolayer of particles and includes a second set of one or more microfluidic channels configured to remove fluid flowing through the filter disposed on the inflatable bladder. The apparatus also includes a pressure source, a sample container connected to the pressure source and the first set of one or more microfluidic channels, a fluid flow gauge configured to measure a fluid flow rate through the filter, and a stain source connected to the first set of one or more microfluidic channels.

L'invention porte sur un appareil de traitement d'un spécimen, provenant d'un échantillon de fluide, qui comprend un premier ensemble d'un ou de plusieurs canaux microfluidiques configurés pour distribuer l'échantillon de fluide à un filtre disposé sur une vessie gonflable configurée pour transférer l'échantillon du filtre à une lame. L'appareil est configuré pour rassembler une monocouche approximative de particules et comprend un second ensemble d'un ou de plusieurs canaux microfluidiques configurés pour évacuer le fluide s'écoulant à travers le filtre disposé sur la vessie gonflable. L'appareil comprend également une source de pression, un contenant d'échantillon relié à la source de pression et le premier ensemble d'un ou de plusieurs canaux microfluidiques, un débitmètre de fluide configuré pour mesurer le débit de fluide à travers le filtre, et une source de coloration reliée au premier ensemble d'un ou de plusieurs canaux microfluidiques.

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