Modified amylase from pseudomonas saccharophilia

Details Modified amylase from pseudomonas saccharophilia Modified amylase from pseudomonas saccharophilia

C - Chemistry, Metallurgy

12

N

C12N 9/28 (2006.01)

Patent

CA 2614274

We describe a PS4 variant polypeptide derivable from a parent polypeptide having amylase activity selected from the group consisting of: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1 , uses of such a polypeptide as a food or feed additive, and nucleic acids encoding such.

L'invention concerne un polypeptide variant PS4 dérivable d'un polypeptide parent ayant une activité d'amylase, choisi dans le groupe constitué de : (a) un polypeptide comprenant une mutation aminoacide à chacune des positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 et 334; (b) un polypeptide comprenant une mutation aminoacide à chacune des positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 et 334; (c) un polypeptide comprenant une mutation aminoacide à chacune des positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 et 334; et (d) un polypeptide comprenant une mutation aminoacide à chacune des positions 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 et 334; par rapport à la numérotation de position d'une séquence exoamylase pseudomonias saccharophilia représentée par SEQ ID N°1. Les utilisations dudit polypeptide en tant qu'additif alimentaire ou pour rations animales, et les acides nucléiques les codant sont également décrits.

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