Modified enzymes and their use for peptide synthesis

Details Modified enzymes and their use for peptide synthesis Modified enzymes and their use for peptide synthesis

A - Human Necessities

61

K

A61K 38/43 (2006.01) A61K 38/46 (2006.01) A61K 38/47 (2006.01) A61K 38/48 (2006.01) C07K 1/02 (2006.01) C07K 5/06 (2006.01) C07K 5/065 (2006.01) C07K 5/068 (2006.01) C07K 5/072 (2006.01) C11D 3/00 (2006.01) C11D 3/386 (2006.01) C12N 9/14 (2006.01) C12N 9/16 (2006.01) C12N 9/48 (2006.01) C12N 9/50 (2006.01) C12N 9/52 (2006.01) C12N 9/54 (2006.01) C12N 9/56 (2006.01) C12N 9/80 (2006.01) C12N 15/00 (2006.01) C12P 21/06 (2006.01) D06M 16/00 (2006.01)

Patent

CA 2321169

The present invention relates to modified enzymes with one or more amino acid residues from an enzyme being replaced by cysteine residues, where at least some of the cysteine residues are modified by replacing thiol hydrogen in the cysteine residue with a thiol side chain to form a modified enzyme, wherein the modified enzyme has high esterase and low amidase activity. Also, a method of producing the modified enzymes is provided. The present invention also relates to a method for using the modified enzymes in peptide synthesis.

La présente invention concerne des enzymes modifiées, un ou plusieurs restes d'acides aminés issus d'une enzyme étant remplacés par des restes de cystéine. Dans ces enzymes, certains des restes de cystéine au moins sont modifiés en remplaçant un hydrogène du thiol dans le reste de cystéine par une chaîne latérale thiolique pour former une enzyme modifiée, l'enzyme modifiée présentant une grande activité estérasique et une faible activité amidasique. L'invention concerne également un procédé permettant de produire ces enzymes modifiées. La présente invention concerne enfin un procédé d'utilisation des enzymes dans la synthèse peptidique.

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