Modulators of anchoring protein function

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/55 (2006.01) A61K 38/08 (2006.01) A61K 38/10 (2006.01) A61K 38/16 (2006.01) C07K 7/06 (2006.01) C07K 7/08 (2006.01) C07K 14/47 (2006.01) C12N 9/16 (2006.01) C12N 15/12 (2006.01) C12Q 1/42 (2006.01) G01N 33/543 (2006.01) G01N 33/566 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2181826

The present invention provides compositions and methods useful for isolating calcineurin as well as inhibiting calcineurin activity. The compositions are peptides that contain regions that are homologous to calcineurin-binding regions of AKAP 79. Also provided are methods for determining if a cell contains a calcineurin-binding and PKA-binding anchoring protein that are useful for identifying additional proteins that bind both calcineurin and PKA. Another aspect of the present invention is methods for enhancing expression of interleukin 2 by T cells.

La présente invention concerne des compositions et des procédés utiles pour isoler la calcineurine ainsi que pour bloquer l'activité de la calcineurine. Les compositions considérées sont des peptides présentant des régions homologues de régions de liaison de la calcineurine de la protéine AKAP 79. L'invention concerne également des procédé permettant de déterminer si une cellule contient un protéine d'ancrage se liant à la calcineurine et se liant à une protéine-kinase, permettant d'identifier les protéines additionnelles se liant aussi bien à la calcineurine qu'à la protéine kinase. L'invention concerne enfin des procédés renforçant l'expression de l'interleukine 2 par les lymphocytes.

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