Mutant aox2 promoter, vector carrying same, transformant,...

Details Mutant aox2 promoter, vector carrying same, transformant,... Mutant aox2 promoter, vector carrying same, transformant,...

C - Chemistry, Metallurgy

12

N

C12N 15/81 (2006.01) C12N 1/19 (2006.01) C12N 15/11 (2006.01) C12N 15/14 (2006.01) C12P 21/02 (2006.01) C12N 15/64 (2006.01) C12N 15/65 (2006.01)

Patent

CA 2102043

Disclosed are (a) a mutant AOX2 promoter obtained by mutating a sequence of natural AOX2 promoter in a manner comprising at least one of the three mutation modes of (1) a region extending upstream from nucleotide 1187 inclusive and comprising at least nucleotides 845-960 is deleted, (2) nucleotide(s) is(are) replaced in region(s) in nucleotides 1274-1314, and (3) new oligonucleotide(s) is(are) inserted in region(s) in nucleotides 1274-1314, (b) a vector carrying the mutant AOX2 promoter, (c) a transformant into which the vector has been introduced, and (d) a method for producing a heterologous protein, by cultivating the transformant. The promoter has remarkably enhanced activity as compared with natural AOX2 promoter, and is highly useful as a promoter to be carried in an expression vector allowing heterologous protein expression. In addition, the vector and the transformant can efficiently express and produce various useful heterologous proteins.

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