Mutant bacterial strains of the genus sphingomomas deficient...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/52 (2006.01) A23L 1/00 (2006.01) A23L 1/054 (2006.01) A23L 1/06 (2006.01) A23L 1/068 (2006.01) C08B 37/00 (2006.01) C12N 1/20 (2006.01) C12N 9/00 (2006.01) C12P 7/62 (2006.01)

Patent

CA 2401179

The invention relates to mutant strains of the genus Sphingomonas which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate ("PHB") synthesis that allows the mutant strains to produce PHB-deficient sphingans. The invention is also directed to a process for preparing a clarified sphingan solution comprising heating aqueous sphingan solution, in particular PHB-deficient sphingan solution, to a clarification temperature of about 30 ~C to about 70 ~C, and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.

L'invention concerne des souches mutantes du genre Sphingomonas présentant une mutation dans un gène codant pour une protéine impliquée dans la synthèse du polyhydroxybutyrate ("PHB"), qui permet aux souches mutantes de produire des sphinganes déficients dans la production de PHB. L'invention concerne également un procédé de préparation d'une solution clarifiée de sphingane consistant à chauffer la solution aqueuse de sphingane, en particulier, la solution de sphingane déficiente dans la production du PHB, à une température de clarification d'environ 30 ·C à 70 ·C; et à traiter ladite solution au moyen d'un agent de clarification et d'enzymes. L'invention concerne, en outre, un produit alimentaire ou industriel contenant un sphingane déficient dans la production du PHB et/ou clarifié. Selon un mode de réalisation particulier, l'invention concerne un gellane clarifié à teneur en acyle élevée, déficient dans la production de PHB et ses procédés de fabrication.

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