Nuclear male sterile plants, method of producing same and...

Details Nuclear male sterile plants, method of producing same and... Nuclear male sterile plants, method of producing same and...

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 9/16 (2006.01) C12N 15/31 (2006.01) C12N 15/55 (2006.01)

Patent

CA 2307956

The present invention is directed to the production of male sterile plants by providing them with a recombinant DNA capable of specific expression in the male reproductive system of a plant of the enzyme trehalose phosphate phosphatase (TPP). Restoration of the fertility can be established either by providing said male sterile plants with a recombinant DNA capable of expression of trehalose phosphate synthase (TPS) under control of an inducible promoter or with a recombinant DNA capable of expression of a suppressor protein which suppresses expression of TPP under control of an inducible promoter. This inducible restoration possibilities enable the maintenance of a homozygous male sterile line. Restoration can also be done by spraying the male sterile plants with gibberellic acid. For production of hybrids or hybrid seed a site-specific recombination system is provided, by inserting two site- specific recombination sites flanking the recombinant DNA coding for TPP and crossing the male sterile lines with lines expressing the corresponding recombinase. By crossing the recombinase will excise the gene coding for TPP and fertile hybrids are produced.

L'invention concerne la production de plantes mâles stériles par l'introduction dans ces plantes d'un ADN recombinant capable d'exprimer l'enzyme tréhalose phosphate phosphatase (TPP) de manière spécifique dans le système reproducteur mâle d'une plante. On peut restaurer la fertilité en introduisant dans plantes stériles mâles soit un ADN recombinant capable d'une expression de tréhalose phosphate synthase (TPS) régulée par un promoteur inductible soit un ADN recombinant capable d'exprimer une protéine suppressive qui bloque l'expression de TPP régulée par un promoteur inductible. Ces possibilités de restauration inductibles permettent de maintenir une lignée mâle stérile homozygote. On peut également restaurer la fertilité en vaporisant les plantes mâles stériles avec de l'acide gibbérellique. Pour produire des hybrides ou de la semence d'hybrides on applique un système de recombinaison spécifique du site, en insérant deux sites de recombinaison spécifiques du site de part et d'autre de l'ADN recombinant codant pour le TPP, et en hybridant les lignées mâles stériles avec des lignées exprimant la recombinase correspondante. Suite à l'hybridation, la recombinase va exciser le gène codant pour la TPP et permettre ainsi la production d'hybrides fertiles.

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