C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/123, 195/39,
C12N 9/86 (2006.01) C12Q 1/34 (2006.01) G01N 33/70 (2006.01)
Patent
CA 1261288
ABSTRACT A nucleoside triphosphate-dependent 1-methyl- hydantoinase, a process for obtaining it and the use thereof The present invention provides 1-methylhydantoin- ase, which hydrolyses 1-methylhydantoin in the presence of a nucleoside triphosphate and of polyvalent metal ions. The present invention also provides a process for obtaining 1-methylhydantoinase and a reagent containing it. Furthermore, the present invention provides a process for the determination of creatinine by the conversion of the creatinine with creatinine deiminase (E.C. 3.5.4.21) into 1-methylhydantoin, hydrolysis of the latter with the 1-methylhydantoinase in the presence of nucleoside triphosphate and of polyvalent metal ions and determination a) of the hydrolysis product formed from l-methyl- hydantoin with N-carbamoylsarcosinamidohydrolase with formation of sarcosine and detection of the sarcosine with sarcosine oxidase or sarcosine dehydrogenase or b) of the simultaneously formed nucleoside diphosphate.
474964
Deeg Rolf
Gauhl Helmgard
Mollering Hans
Roder Albert
Siedel Joachim
Boehringer Mannheim G.m.b.h.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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