Nucleotide and amino acid sequences of a t. pallidum rare...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/31 (2006.01) A61K 39/02 (2006.01) C07K 14/20 (2006.01) C07K 16/12 (2006.01) C12Q 1/68 (2006.01) G01N 33/564 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2180427

Antigenic rare outer membrane proteins of Spirochaetaceae are obtained from organisms extracted from infected tissue by a novel process of isolation utilizing a discontinuous Ficoll gradient separation, release of outer membrane in a low isotonic and low pH buffer and identification of outer membrane by use of a lipid soluble dye. Four antigenic rare outer membrane proteins of T.pallidum subsp. pallidum useful in diagnosis and prophylaxis of syphilis are provided. Also provided is the amino acid sequence of a rare outer membrane protein of T. pallidum subsp. pallidum and the nucleotide sequence encoding it.

Des protéines antigéniques rares de la membrane extérieure de la Spirochaetaceae sont obtenues à partir d'organismes extraits de tissus infectés. L'extraction se fait grâce à un nouveau procédé d'isolation utilisant une séparation à gradient de Ficoll, ce procédé permettant, d'une part de libérer la membrane extérieure dans un tampon à isotonicité et pH bas, et d'autre part d'identifier la membrane extérieure grâce à une teinture liposoluble. L'invention concerne également quatre protéines antigéniques rares de la sous-espèce pallidum du T. pallidum utiles pour le diagnostic et la prophylaxie de la syphilis. L'invention concerne en outre la séquence d'acides aminés d'une protéine rare de la membrane extérieure de la sous-espèce pallidum du T. pallidum ainsi que la séquence de nucléotides codant cette protéine.

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