Nucleotide sequences which code for the opca gene

Details Nucleotide sequences which code for the opca gene Nucleotide sequences which code for the opca gene

C - Chemistry, Metallurgy

12

N

C12N 15/53 (2006.01) C12N 1/21 (2006.01) C12N 9/04 (2006.01) C12N 9/10 (2006.01) C12P 13/06 (2006.01) C12P 13/08 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2348365

The invention relates to an isolated polynucleotide from coryneform bacteria, comprising at least one polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for polypeptides which comprise at least one of the amino acid sequences according to SEQ ID No. 3 or SEQ ID No. 5 or SEQ ID No. 8 or SEQ ID No. 10, b) polynucleotide which codes for polypeptides which comprise amino acid sequences which are identical to the extent of at least 70 % to the amino acid sequences according to SEQ ID No. 3 or SEQ ID No. 5 or according to SEQ ID No. 8 or SEQ ID No. 10, c) polynucleotide which is complementary to the polynucleotides of a) or b), or d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequences of a), b) or c), and a process for the fermentative preparation of L-amino acids, which comprises in the coryneform microorganisms which in particular already produce L-amino acids, a) amplifying, in particular over-expressing, in addition to the opcA gene, at least one of the nucleotide sequences which code for the tal gene, tkt gene, zwf gene or the devB gene, b) concentrating the desired L-amino acid in the medium or in the cells of the bacteria and c) isolating the L-amino acid.

L'invention concerne un polynucléotide isolé de bactéries du type coryne, comprenant au moins une séquence polynucléotidique choisie dans le groupe comprenant a) un polynucléotide identique à au moins 70 % à un polynucléotide codant pour des polypeptides constitués d'au moins une séquence d'acide aminé correspondant aux numéros d'identification SEQ ID No. 3, SEQ ID No. 5, SEQ ID No. 8 ou SEQ ID No. 10; b) un polynucléotide codant pour des polypeptides constitués de séquences d'acide aminé identiques à au moins 70 % aux séquences d'acide aminé correspondant aux numéros d'identification SEQ ID No. 3 ou SEQ ID No. 5, ou correspondant aux numéros d'identification SEQ ID No. 8 ou SEQ ID No. 10; c) un polynucléotide complémentaire aux polynucléotides de a) ou de b); ou d) un polynucléotide constitué d'au moins 15 nucléotides successifs des séquences polynucléotidiques de a), de b) ou de c); ainsi qu'un processus de préparation de la fermentation de L-aminoacides qui consiste, dans les micro-organismes du type coryne qui, en particulier, produisent déjà des L-aminoacides, a) à amplifier, en particulier surexprimer, outre le gène opcA, au moins une des séquences nucléotidiques codant pour le gène tal, le gène tkt, le gène zwf ou le gène devB; b) à concentrer l'acide aminé voulu dans le milieu ou dans les cellules des bactéries; et c) à isoler Le L-aminoacide.

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