O-linked glycosylation of peptides

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/27 (2006.01) A61K 38/14 (2006.01) A61K 38/19 (2006.01) A61K 38/21 (2006.01) A61K 38/27 (2006.01) A61P 5/00 (2006.01) C07K 14/00 (2006.01) C07K 14/535 (2006.01) C07K 14/56 (2006.01) C07K 14/61 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/18 (2006.01) C12N 15/21 (2006.01) C12N 15/63 (2006.01) C12P 19/00 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2552892

The present invention provides polypeptides that include an O-linked glycosylation site that is not present in the wild-type peptide. The polypeptides of the invention include glycoconjugates in which a species such as a water-soluble polymer, a therapeutic agent of a biomolecule is covalently linked through an intact O-linked glycosyl residue to the polypeptide. Also provided are methods of making the peptides of the invention and methods, pharmaceutical compositions containing the peptides and methods of treating, ameliorating or preventing diseased in mammals by administering an amount of a peptide of the invention sufficient to achieve the desired response.

La présente invention concerne des polypeptides qui comprennent un site de glycosylation liée à O qu'on ne trouve pas dans le peptide de type sauvage. Les polypeptides de cette invention comprennent des glycoconjugués dans lesquels une espèce telle qu'un polymère soluble dans l'eau, un agent thérapeutique d'une biomolécule, est liée par covalence au polypeptide par le biais d'un résidu glycosyl intact lié à O. Cette invention concerne également des procédés de production des peptides de cette invention ainsi que des procédés, des compositions pharmaceutiques contenant ces peptides et des méthodes permettant de traiter, d'améliorer ou de prévenir des maladies chez des mammifères et consistant à administrer un peptide de cette invention en quantité suffisante pour qu'on obtienne la réponse voulue.

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