Oligonucleotide sizing using cleavable primers

Details Oligonucleotide sizing using cleavable primers Oligonucleotide sizing using cleavable primers

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2220418

The present invention provides modified oligonucleotide primers designed to incorporate a cleavable moiety so that a (3') portion of the primer (linked to an extension product) can be released from an upstream (5') portion of the primer. Upon selective cleavage of the cleavable site, primer extension products that contain about five or fewer base pairs of the primer sequence are released, to provide more useful sizing and sequence information per fragment than extension products containing the entire primer.

La présente invention concerne des amorces oligonucléotidiques modifiées comportant une fraction scindable de sorte qu'une portion (3') de l'amorce (fixée à un produit d'allongement) puisse être libérée d'une portion (5') d'amont de l'amorce. Lors de la scission sélective du site scindable, les produits d'allongement de l'amorce qui contiennent environ cinq paires de bases ou moins de la séquence de l'amorce se trouvent libérés, ce qui permet une détermination plus utile de la taille et des séquences par fragment qu'avec les produits d'allongement contenant toute l'amorce.

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