G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/543 (2006.01) G01N 21/77 (2006.01)
Patent
CA 2183204
A new optical solid-phase biosensor based on the Förster energy transfer betweena fluorescent dye F1 and F2, for example in the form of a test strip, consists of a) a transparent support, b) an adjacent multilayer of polyanions and polycations which, as uppermost layer, contains biotinylated polylysine hydrobromide, c) a covering of the uppermost ionic layer by streptavidin, which is bound to the biotinylated layer, and d) further biotinylated biomolecules as receptors, it being possible for the fluorescent dye F1 to be bound to the streptavidin or to other biomolecules. The analyte is furnished with the dye F2 or competes with an analyte furnished with F2 and bound to the biosensor.
Diederich Anke
Heiliger Ludger
Losche Matthias
Siegmund Hans-Ulrich
Volker Michael
Aktiengesellschaft Bayer
Fetherstonhaugh & Co.
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