Optimizing expression of active botulinum toxin type a

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/31 (2006.01) A61K 38/16 (2006.01) A61K 38/48 (2006.01) A61K 39/08 (2006.01) C07K 14/33 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/63 (2006.01) C12N 9/52 (2006.01)

Patent

CA 2575994

Nucleic acid molecules that comprise modified open reading frames providing increased expression of the encoded active BoNT/A in a heterologous cell, expression constructs and cells comprising such nucleic acid molecules and methods useful for expressing the encoding active BoNT/A from such nucleic acid molecules, expression constructs and cells.

La présente invention a trait à des molécules d'acide nucléique comportant des cassettes de lecture ouvertes assurant une expression accrue de la BoNT/A active codée dans une cellule hétérologue, à des constructions d'expression et à des cellules comprenant de telles molécules d'acide nucléique et à des procédés pour l'expression du codage de la BoNT/A active à partir de telles molécules d'acide nucléique, constructions d'expression et cellules.

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