Oxidatively stable alpha-amylase

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/56 (2006.01) C11D 3/386 (2006.01) C12N 9/28 (2006.01) C12N 15/75 (2006.01) C12P 19/14 (2006.01)

Patent

CA 2155831

Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha- amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed an detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.

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