Pcr hot start by magnesium sequestration

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C07K 5/113 (2006.01) C07K 7/06 (2006.01) C07K 7/08 (2006.01)

Patent

CA 2638854

The present invention is directed to Synthetic peptide having a length of not more than 30 amino acids comprising a divalent cation binding site. Such a peptide according to the present invention is part of a composition for nucleic acid amplification and provides for a so called hot start effect.

La présente invention concerne un peptide synthétique dont la longueur ne dépasse pas 30 acides aminés et qui comprend un site de liaison de cation divalent. Un tel peptide selon la présente invention fait partie d'une préparation destinée à l'amplification des acides nucléiques et permet d'obtenir l'effet appelé <= hot start >=.

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