Pentose fermentation by recombinant zymomonas

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 1/21 (2006.01) C12N 15/11 (2006.01) C12P 7/06 (2006.01) C12P 7/10 (2006.01)

Patent

CA 2173793

The invention relates to microorganisms which normally do not ferment pentose sugar and which are genetically altered to fermen pentose sugar to produce ethanol, and fermentation processes utilizing the same. Examples include Zymomonas mobilis which has been transformed with combinations of E. coli genes for xylose isomerase, xylulokinase, transaldolase, transketolase, L-arabinose isomerase, L- ribulokinase, and L--ribulose 5-phosphate 4-epimerase. Expression of the added genes are under the control of Zymomonas mobilis promoters. These newly created microorganisms are useful for fermenting pentoses and glucose, produced by hydrolysis of hemicellulose and cellulose, to produce ethanol.

L'invention porte sur des microorganismes qui, généralement, ne transforment pas le pentose par fermentation et qui ont été génétiquement modifiés pour transformer le pentose afin de produire de l'éthanol, et sur des procédés de fermentation faisant appel à ceux-ci. Parmi les exemples figure Zymomonas mobilis, qui a été modifié avec des combinaisons de gènes d'E. coli codant la xylose isomérase, la xylulokinase, la transaldolase, la transcétolase, la L-arabinose isomérase, la L-ribulokinase et la L-ribulose 5-phosphate 4-épimerase. L'expression des gènes ajoutés est régulée par des promoteurs de Zymomonas mobilis. Ces microorganismes nouvellement créés sont utiles pour fermenter le pentose et le glucose, lesquels sont produits par une hydrolyse de l'hémicellulose et de la cellulose, dans le but de produire de l'éthanol.

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