Phencyclidine and phencyclidine metabolites assay, tracers,...

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G01N 33/533 (2006.01) A61K 39/395 (2006.01) C07D 295/033 (2006.01) C07D 493/10 (2006.01) C07K 14/435 (2006.01) C07K 16/44 (2006.01) G01N 33/94 (2006.01)

Patent

CA 2043372

Case No. 4300.U5.P1 PHENCYCLIDINE AND PHENCYCLIDINE METABOLITES ASSAY, TRACERS, IMMUNOGENS, ANTIBODIES AND REAGENT KIT ABSTRACT OF THE DISCLOSURE The present invention is directed to a fluorescence polarization assay for phencyclidine and phencyclidine derivatives, to the various components needed for preparing and carrying out such an assay, and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for making them, and a reagent kit containing them. The tracers and the immunogens are made from substituted phencyclidine compounds. A fluorescein moiety is included in the tracer, while a poly(amino acid) forms a part of the immunogen. The assay is conducted by measuring the degree of polarization retention of plane polarized light that has been passed through a sample containing antiserum and tracer.

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