Polypeptides involved in spiramycin biosynthesis, nucleotide...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/52 (2006.01) C07K 14/36 (2006.01) C12N 1/21 (2006.01) C12N 9/00 (2006.01) C12N 15/63 (2006.01) C12P 19/62 (2006.01)

Patent

CA 2501445

The invention concerns isolation and identification of novel genes of spiramycin biosynthesis process and novel polypeptides involved in said biosynthesis. The invention also concerns a method for producing sand polypeptides. The invention further concerns the use of said genes for increasing the rate of production and purity in the resulting spiramycin. The invention concerns in particular a micro-organism producing spiramycin I but not producing spiramycin II and III and the use of such a micro-organism. The invention also concerns the use of genes in spiramycin biosynthesis process for constructing mutants capable of leading to synthesis of novel antibiotics or derivative forms of spiramycin. Finally the invention concerns molecules produced through expression of said genes and phamacologically active compositions of one molecule produced through expression of such genes.

La présente invention concerne l'isolement et l'identification de nouveaux gènes de la voie de biosynthèse des spiramycines et de nouveaux polypeptides impliqués dans cette biosynthèse. L'invention est également relative à un procédé de production de ces polypeptides. Elle est également relative à l'utilisation de ces gènes dans le but d'augmenter les taux de production et la pureté de la spiramycine produite. L'invention concerne notamment un microorganisme produisant de la spiramycine I mais ne produisant pas de spiramycine II et III et l'utilisation d'un tel micoorganisme. L'invention a également trait à l'utilisation des gènes de la voie de biosynthèse des spiramycines pour la construction de mutants pouvant conduire à la synthèse de nouveaux antibiotiques ou à des formes dérivées de spiramycines. L'invention concerne encore les molécules produites grâce à l'expression de ces gènes et des compositions pharmacologiquement actives d'une molécule produite grâce à l'expression de tels gênes_________________________

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