Preparation of albumin

Details Preparation of albumin Preparation of albumin

A - Human Necessities

23

J

530/15.06

A23J 1/06 (2006.01) C07K 14/765 (2006.01)

Patent

CA 1105382

PREPARATION OF ALBUMIN ABSTRACT OF THE DISCLOSURE Methods for the large scale preparation of clinical albumin are disclosed. One method includes dilution of plasma with a NaCl solution containing disodium ethylene dinitrilo tetraacetate and a stabilizer such as sodium caprylate. The resulting solution is then subjected to heating and cooling steps, followed by the addition of polyethylene glycol to precipitate impurities, with albumin remaining in the supernatant. Isoelectric pre- cipitation is then employed to recover the desired albumin product, which is devoid of the albumin dimer. In an alternative method, ethanol is employed as the precipitating agent rather than polyethylene glycol.

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