Probe for mass spectrometry

Details Probe for mass spectrometry Probe for mass spectrometry

H - Electricity

01

J

H01J 49/04 (2006.01) A61K 47/48 (2006.01) C07K 14/00 (2006.01) G01N 33/94 (2006.01) H01J 49/16 (2006.01)

Patent

CA 2477563

The present invention relates to a probe for the analysis of one or more analytes, particularly proteins or compounds capable of binding or otherwise interacting therewith, by laser desorption/ ionisation mass spectrometry, more particularly MALDI MS. It also relates to a protein microarray, a method of producing a protein microarray and a method of analysing a protein microarray. The probe comprises a support having an electroconductive target surface thereon characterized in that the target surface comprises a micro array having a plurality of discrete target areas presenting one or more analyte capture moieties. Each discrete target area has an area of less than 1000~m2, more preferably still less than 500~m2, and more preferably still less than 100~m2.

L'invention concerne une sonde destinée à l'analyse d'un ou de plusieurs analytes, en particulier de protéines ou de composés pouvant se lier ou interagir avec cette sonde, par spectrométrie de masse d'ionisation/désorption laser, plus particulièrement par spectrométrie de masse MALDI. Elle concerne aussi un jeu ordonné de micro-échantillons protéiques, un procédé permettant de le produire et un procédé d'analyse l'utilisant. La sonde comprend un support comportant une surface cible électroconductrice caractérisé en ce que la surface cible comprend un jeu ordonné de micro-zones cibles discrètes présentant une ou plusieurs entités de capture d'analyte. Chaque zone cible discrète possède une surface inférieure à 1000µm<sp>2</sp>, de préférence inférieure à 500µm<sp>2</sp>, et préférablement inférieure à 100µm<sp>2</sp>.

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