C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/3.2, 150/15.
C12Q 1/52 (2006.01)
Patent
CA 1206401
ABSTRACT Process and analytical agent for the determination of the activity of glutamate-oxalacetate- transaminase The present invention provides a process for the determination of the activity of glutamate- oxalacetate-transaminase (GOT) in aqueous solutions in which a) .alpha.-ketoglutarate is reacted with alanine-sulphinic acid, catalysed by GOT, to give pyruvate, sulphur dioxide and glutamate, b) the resultant pyruvate is reacted with phosphate and oxygen, catalysed by pyruvate oxidase, to give acetyl phosphate, carbon dioxide and hydrogen pexoxide, c) the hydrogen peroxide and a chromogen is reacted, catalysed by a peroxidase, to give a dyestuff and water and d) the amount of dyestuff formed is determined, wherein the chromogen used is N-(3'-sulphoxlyl-4'- hydroxy-5'-chlorophenyl)-4-aminoantipyrine (SHYC-AA), 4,5-bis-[4-dimethylaminophenyl]-2-[3,5-dimethoxy-4- hydroxyphenyl]-imidazole (B-DDH-imidazole) or a related substance. The present invention also provides an analytical agent for the determination of the activity of GOT, comprising .alpha.-ketoglutarate, alanine-sulphinic
429972
Batz Hans-Georg
Deneke Ulfert
Gruber Wolfgang
Boehringer Mannheim G.m.b.h.
Marcoux & Sher Swabey Mitchell Houle
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