Process and device for specific binding assay

G - Physics – 01 – N

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G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/58 (2006.01) G01N 33/74 (2006.01) G01N 33/76 (2006.01)

Patent

CA 2069833

ABSTRACT OF THE DISCLOSURE An assay process and a device useful for the practice of the assay process, effective for high sensitivity semi- quantitative assay, in which assay results can be obtained by simply loading a sample as it is and any desired detection sensitivity can be selected at will without causing decrease (or increase) in the maximum intensity of signals. Particularly, this invention provides a process for specific binding assay in which a substance in a test sample to be assayed is detected qualitatively or quantitatively making use of specific binding reaction, which comprises including a specified substance in an assay system and, by the effect of the presence of the specified substance, decreasing (or increasing) amounts of a labeled material to be measured as an index of the amount of the substance to be assayed. It also provides a chromatography type specific binding assay device which comprises a means for loading a test sample, a means for locating specific binding substances, a means for locating a detecting element and a means for absorbing liquid materials in that order, wherein a specific binding substance and a labeled specific binding substance are included in the means for locating specific binding substances and a detecting element on which a specific binding substance is immobilized is included in the means for locating a detecting element.

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