Process for concentrating and processing fluid samples

Details Process for concentrating and processing fluid samples Process for concentrating and processing fluid samples

B - Operations, Transporting

01

D

B01D 61/00 (2006.01) C12Q 1/04 (2006.01) C12Q 1/24 (2006.01) G01N 33/487 (2006.01)

Patent

CA 2728331

A method of treating a liquid sample having microbiological target species therein to concentrate the species and collect lysate is disclosed. The liquid sample comprises non-target microbiological particles, inorganic particles, and microbiolog-ical target species. The liquid is passed through a prefilter medium to allow the target species to pass through as filtrate and retain non-target microbiological products and inorganic particles thereon. The filtrate is contacted with a main filtration medium adapt-ed to retain the target species thereon as retentate. The retentate is lysed to form a lysate containing target material that was en-veloped within the microbiological target species. The microbiological target species may comprise cellular organisms such as bacteria, algae, fungi, prokaryotes, etc. or viruses. Target materials comprise intracellular nucleic acids, or in the case of viral sam-pling, nucleic acids encased within the protein sheath or coating of the virus.

L'invention concerne un procédé de traitement d'un échantillon liquide contenant des espèces cibles microbiologiques, permettant de concentrer les espèces et de collecter le lysat. L'échantillon liquide contient des particules microbiologiques non cibles, des particules inorganiques et des espèces cibles microbiologiques. Le liquide est passé à travers un milieu de préfiltre qui permet aux espèces cibles de le traverser en tant que filtrat et qui retient les produits microbiologiques non cibles et les particules inorganiques. Le filtrat est mis en contact avec un milieu de filtration principal conçu pour retenir les espèces cibles en tant que rétentat. Ce rétentat est lysé pour former un lysat contenant une matière cible enveloppée dans les espèces cibles microbiologiques. Ces dernières peuvent comprendre des organismes cellulaires tels que des bactéries, des algues, des champignons, des procaryotes, etc. ou des virus. Les matières cibles comprennent des acides nucléiques intracellulaires ou, dans le cas de l'échantillonnage viral, des acides nucléiques enrobés dans la gaine protéique ou l'enveloppe du virus.

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