C - Chemistry – Metallurgy – 07 – H
Patent
C - Chemistry, Metallurgy
07
H
C07H 21/00 (2006.01) C01B 21/068 (2006.01) C12N 1/08 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/553 (2006.01)
Patent
CA 2481481
A process for isolating nucleic acid from a nucleic acid-containing sample, which comprises: (a) providing a chaotrope; (b) providing a nucleic acid binding solid phase capable of binding nucleic acid in the presence of the chaotrope; (c) providing a source of NH4+ or NH3; (d) contacting the sample with the nucleic acid binding solid phase in the presence of a liquid phase comprising the chaotrope and the NH4+ or NH3; and (e) optionally separating the solid phase with the nucleic acid bound thereto from the liquid phase.
L'invention concerne un procédé permettant d'isoler de l'acide nucléique à partir d'un échantillon contenant de l'acide nucléique. Ledit procédé comprend les étapes suivantes : (a) fournir un chaotrope ; (b) fournir une phase solide liant l'acide nucléique, apte à lier l'acide nucléique en présence du chaotrope ; (c) fournir une source de NH¿4??+¿ ou de NH¿3 ?; (d) mettre en contact l'échantillon avec la phase solide liant l'acide nucléique en présence d'une phase liquide comprenant le chaotrope et le NH¿4??+¿ ou le NH¿3 ?; et (e) éventuellement séparer la phase solide de la phase liquide, conjointement avec l'acide nucléique qui lui est lié.
Deggerdal Arne
Reitan Evy
Skagestad Vidar
Fetherstonhaugh & Co.
Qiagen As
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