Process for producing bovine lactoferrin in high purity

Details Process for producing bovine lactoferrin in high purity Process for producing bovine lactoferrin in high purity

A - Human Necessities

23

J

530/15.06, 530/1

A23J 1/20 (2006.01)

Patent

CA 1323465

A B S T R A C T A process for producing bovine lactoferrin in high purity from raw milk-materials containing skim milk or whey originated from cow's milk which comprises: adsoption step wherein said raw materials are contacted, at a temperature between 0 - 60 °C, with weakly acidic cation-exchanger which includes carboxymethyl groups as ion exchanging groups and has haemoglobin adsorption property more than 3.5 g/ 100 m?; rinsing step wherein said exchanger is washed with water to remove substances other than those adsorbed to said exchanger; and desorption step wherein the substances adsorbed to said exchanger are desorbed therefrom with a solution of one or more of salts to thereby yield highly purified bovine lactoferrin without deterirating raw milk materials. Said desorption step can be carried out in a single stage to produce lactoferrin more than 80 % purity to total proteins recovered, and in two stages to attain more than 98 % purity.

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