Process for promoting the differenciation of plant cells...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/29 (2006.01) A01H 4/00 (2006.01) A01H 5/00 (2006.01) C07H 21/00 (2006.01) C07K 14/415 (2006.01) C12N 5/00 (2006.01) C12N 5/02 (2006.01) C12N 5/04 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2151587

The invention relates to a method for enhancing the differentiation of cells in culture, particularly for the tissue differentiation, embryogenesis and/or organogenesis, characterized in that at least one lipid transfer protein or "LTP" or an LTP analog is introduced in the culture medium in a concentration efficient to obtain the cell differentiation. The invention also relates to a plant obtained by such a method, proteinic substances and proteins having a lipid transfer activity, and a composition comprising at least such substance. It also relates to a DNA sequence coding for an LTP and a probe whose sequence is deduced from the sequence of said protein.

La présente invention concerne un procédé pour favoriser la différenciation de cellules en culture, notamment en vue de la différenciation tissulaire, de l'embryogénèse et/ou de l'organogénèse, caractérisé en ce qu'on introduit dans le milieu de culture en concentration efficace pour obtenir la différenciation des cellules, au moins une protéine de transfert de lipide ou "LTP" ou un analogue de LTP. Elle concerne également une plante obtenue par un tel procédé, des substances protéiques et protéines possédant une activité de transfert de lipides et une composition comportant au moins une telle substance. Elle concerne enfin une séquence d'ADN codant pour une LTP et une sonde dont la séquence est déduite de la séquence de ladite protéine.

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