C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
530/13, 195/71,
C07K 14/55 (2006.01) C07K 1/113 (2006.01) C07K 1/36 (2006.01) C12N 15/26 (2006.01)
Patent
CA 1337671
A process for recovering highly pure, recombinant IL-2 from transformed microorganisms in which the cells are disrupted; impure recombinant IL-2 is isolated in the form of refractile bodies from the disruptate; the impure IL-2 is dissolved and denatured with at least 6 M guanidine hydrochloride containing a reducing agent; the reduced IL-2 is precipitated and resolubilized; the reduced solubilized IL-2 therein is oxidized by a controlled oxidation in which the con- centration of guanidine hydrochloride is maintained at least at 6 M; the oxidized IL-2 is refolded by reducing the concentration of guanidine hydrochloride in the solution; and the oxidized, refolded IL-2 is further purified by ion exchange chromatography or hydrophobic interaction chromatography and ion exchange chroma- tography.
563757
Davis John T.
Dorin Glenn
Lim Amy
Smith Flint
Wolfe Sidney N.
Borden Ladner Gervais Llp
Novartis Vaccines And Diagnostics Inc.
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