A - Human Necessities – 23 – J
Patent
A - Human Necessities
23
J
93/72, 195/93
A23J 1/18 (2006.01) C12N 1/08 (2006.01) C12N 1/16 (2006.01) C12N 9/22 (2006.01)
Patent
CA 1079568
ABSTRACT This disclosure relates to a process for producing a yeast protein by treating the yeast medium with zinc at critical points in the process to protect the endogenous nuclease when the pH of the medium is raised during separation of the protein from the cell walls. The endogenous nuclease then is available to reduce the nucleic acid content of the protein during a sub- sequent incubation step. The yeast cells are ruptured by homogenization; ex- tracted to remove the protein from the cells; and the cell wall debris separated by centrifugation. The soluble fraction is treated with alkali to enable the endogenous ribonuclease to de- grade the nucleic acid. Specifically it has been found that adding zinc to the growing yeast, or to the cells before homo- genization, or to the homogenate of cell walls and yeast pro- tein before it is made highly alkaline will permit the endogenous nuclease of the yeast to degrade its own nucleic acid to toler- able levels.
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