Process for the isolation of proteins from rapeseed

Details Process for the isolation of proteins from rapeseed Process for the isolation of proteins from rapeseed

A - Human Necessities

23

J

530/15.1

A23J 1/14 (2006.01)

Patent

CA 1086307

Abstract of the disclosure A countercurrent procedure for the extraction of protein from defatted rapeseed meal is described. Using 0.02 N NaOH and a meal to solvent ratio of 1:25 as much as 94 % of meal nitrogen is extracted. A two step precipitation, first at pH 6.0 and then at pH 3.6, the two isoelectric points of the protein in the extract, affords two protein fractions, which contain 69.4 % and 24.3 % of the meal protein, respectively. After washing the curds with water and drying with acetone two highly pure isolates are obtained. Protein Isolate I, light grey, contains 92.9 % protein and Protein Isolate II, white, contains 98.6 % protein, both on a dry weight basis. These protein isolates are light colored, bland products having a foam stability higher than soybean protein. Their amino acid composition shows adequate amounts of isoleucine and sulphur amino acids. Nutritional experiments prove better performance of the protein isolates as compared to the corres- ponding meals. - 1 -

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