C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 17/16 (2006.01) C12N 1/20 (2006.01) C12P 17/02 (2006.01)
Patent
CA 2360865
A procedure for the preparation of pseudomonic acid A comprising submerged cultivation of a Pseudomonas bacterium strain capable of biosynthesis of the substantially pure pseudomonic acid A in aerated conditions via fermentation; and isolation of the desired compound is disclosed. In particular, the procedure of the present invention comprises cultivation of the Pseudomonas sp. bacterium strain No. 19/26 deposited under accession No. NCAIM(P)B 001235 in the National Collection of the Agricultural and Industrial Microorganisms, Budapest, Hungary, or its pseudomonic acid A-producing mutant or variant, on a medium at a temperature of between about 20 ~C and 30 ~C containing organic nitrogen and carbon sources and, optionally, mineral salts.
La présente invention concerne un procédé de préparation d'acide A pseudomonique comprenant la culture submergée d'une souche de la bactérie Pseudomonas capable de biosynthèse de l'acide A pseudomonique sensiblement pure dans des conditions d'aération par fermentation. L'invention concerne également l'isolement du composé désiré. En particulier, le procédé de la présente invention comprend la culture de la souche de la bactérie Pseudomonas sp. No. 19/26 déposée sous le No. d'entrée NCAIM(P)B 001235 dans la Collection Nationale des Microorganismes Agricoles et Industriels, Budapest, Hongrie, ou de son mutant ou variant producteur d'acide A pseudomonique, sur un milieu à une température située entre 20 ·C et 30 ·C environ, contenant de l'azote organique et des sources de charbon et, éventuellement, des sels minéraux.
Albrecht Karoly
Balogh Gabor
Barta Istvan
Erdei Janos
Gulyas Eva
Biogal Gyogyszergyar Rt.
Ridout & Maybee Llp
Teva Gyogyszergyar Reszvenytarsasag
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