Process of controlled shuffling of chromosome fragments for...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/11 (2006.01) A01H 5/00 (2006.01) C12N 15/10 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2445457

Disclosed is a method of constructing a wild-species genomic library of chromosome fragments incorporated in a crop-species genome. First, a number of transformants for donor and recipient plant species is produced, carrying the DNA constructs necessary for the exchange of chromosomal fragments mediated by site-specific recombination. The donor and recipient are chosen such that, upon sexual cross or somatic cell fusion, they produce unstable progeny or demonstrate preferential segregation or sorting out. Second, the crossing between donor and recipient species and formation of chromosomal recombinants of donor and recipient plant species is induced. Third, taking advantage of the instability of hybrids between donor and recipient, recombinant cells and plants of the recipient are selected which contain specific chromosome fragments of the donor species. Also disclosed are transgenic plants, libraries and breeding material produced by the methods.

L'invention porte sur un procédé de constitution d'une bibliothèque de génomes de fragments de chromosomes d'espèces sauvages incorporés à un génome de plante de culture comportant les étapes suivantes: (1) élaboration de transformants des donneurs et receveurs d'espèces végétales porteurs des gènes hybrides nécessaires à l'échange de fragments de chromosomes médiés par un recombinaison spécifique au niveau du site, le donneur et le receveur étant choisis pour donner une descendance par croisement sexuel ou fusion cellulaire somatique, ou présenter une ségrégation préférentielle, ou être obtenus par triage; (2) induction du croisement des espèces donneuses et receveuses et formation de chromosomes de recombinaison des espèces donneuses et receveuses; et (3) mettant à profit l'instabilité des hybrides entre donneurs et receveurs, sélection de cellules et de plantes de recombinaison du receveur contenant des fragments spécifiques de chromosomes de l'espèce donneuse. L'invention porte également sur des plantes transgéniques, sur des bibliothèques, et sur du matériel de sélection obtenus par lesdits procédés.

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