C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/96 (2006.01) C07K 1/113 (2006.01) C07K 1/22 (2006.01) C07K 1/30 (2006.01) C07K 14/60 (2006.01) C07K 14/605 (2006.01) C07K 14/635 (2006.01) C12N 9/88 (2006.01) C12P 21/02 (2006.01) C12P 21/06 (2006.01)
Patent
CA 2206848
A method for the isolation and/or purification of a recombinant peptide by employing a fusion protein construct which includes a carbonic anhydrase and a variable fused polypeptide is provided. The method includes precipitating either the fusion protein construct or a fragment of the fusion protein construct including the carbonic anhydrase. Inclusion bodies which includes the fusion protein construct and a method of producing a peptide which includes expressing the fusion protein construct as a part of an inclusion body are also provided. Fusion protein constructs which include a carbonic anhydrase and certain target peptides are also provided.
L'invention décrit un procédé pour isoler et/ou purifier un peptide de recombinaison à l'aide d'une protéine de fusion de synthèse incluant une anhydrase carbonique et un polypeptide fusionné variable. Ce procédé comporte la précipitation, soit de la protéine de fusion de synthèse, soit d'un fragment de celle-ci incluant l'anhydrase carbonique. L'invention décrit aussi des corps d'inclusion qui incluent la protéine de fision de synthèse et un procédé de production d'un peptide incluant l'expression de la protéine de fusion de synthèse comme faisant partie d'un corps d'inclusion. Les protéines de fusion de synthèse qui incluent une anhydrase carbonique et certains peptides cibles sont également décrits.
de La Motte Rebecca S.
Henriksen Dennis B.
Holmquist Barton
Manning Shane D.
Partridge Bruce E.
Bereskin & Parr
Bionebraska Inc.
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